Reporter

Part:BBa_J70659

Designed by: Julie Norville   Group: Knight Lab   (2010-12-20)


N-terminal 6 strep fusion test for GFP J70610w n-terminalstreptag inserts

This is a version of J70610 that has been cut with BsaXI and had the following insert added

forwardn-terminalstreptag

5'- AGG AGA AAT ACT AGA TGT GGA GCC ACC CGC AAT TCG AGA AAT CTG GTT CCG GT T CTC GTA -3'

reversen-terminalstreptag

5'- GAG AAC CGG AAC CAG ATT TCT CGA ATT GCG GGT GGC TCC ACA TCT AGT ATT TC T CCT CTT -3'


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 875


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Parameters
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