Measurement

Part:BBa_J70604

Designed by: Julie Norville   Group: Knight Lab   (2010-06-28)


J70589 cut with BsaXI and with rbs, 6his inserts (J70559-f1i,f2i,r2i,r2i) added

This tests adding a protein fusion to the n-terminus of a protein using J70589 as a test case.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 863


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Categories
Parameters
None