Part:BBa_J70485
gfp RBS, {0,5;15,10} family member - level 100 (master sequence)
These oligo parts can be used for converting any pathway of the form (Promoter X)-(Bioscaffold part {0,5;5,0})-GFP to (Promoter X)-(RBS level Y)-GFP.
Note that this removes the scar on the right side of the RBS, except when traditional BioBrick derived RBSes have been used (for example BBa_B0034.)
(In this case it was not necessary to remove gtg starts since they were not present near the start of gfp.)
Form of insert:
{promoter} ta ctaga ^ g {rbs master sequence} atg c gtaaa ^ {rest of GFP (E0040)}
{promoter} at ^ gatct c {rbs master sequence} tac g^ cattt {rest of GFP (E0040)}
Forward oligo:
g {rbs master sequence} atg c gtaaa
Reverse oligo:
Reverse complement of (ctagag {rbs master sequence} atg c)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
None |