Temporary

Part:BBa_J70060

Designed by: Julie Norville   Group: Knight Lab   (2009-02-05)

BBa_J70010 (PpiI) protein fusion test part

This is used to test whether J70010 (PpiI) can be used to make protein fusions (can also be cut with BamHI.) 

After excision <Prefix w/AT>G protein G GATCC^                  ^ protein TAA<Suffix>
                                      C^                  ^CTAGG
PpiI recognition/cut sites
5'-^ 7(N) G A A C (N)5 C T C (N)13^-3'
3'-^12(N) C T T G (N)5 G A G (N)8 ^-5'

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 22
    Illegal BamHI site found at 96
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 83


[edit]
Categories
Parameters
None