Tag

Part:BBa_J70048

Designed by: Julie Norville   Group: Knight Lab   (2009-02-05)

6his part for use with BioScaffold parts ATG-GGATCC (glycine serine)-6 His-TAA

See parts J70070, J70074, J70100, and J70104.

This 6 His suffix tag can be used with BBa_J70012 (PsrI) in fusing a 6 his tag to the C-terminus of a protein. This part is still in testing. During the BioScaffold part excision, it should be placed in pSB2K4.

Oligos needed for annealing the part and placing it into a XbaI/PstI cut vector such as pSB2K4 containing J70012 or J70040: 

ctagatgggatcccaccatcaccatcatcattaatactagtagcggccgc tgca^g
    t                                             ^

These oligos look like they are the incorrect length Forward: ctagatgggatcccaccatcaccatcatcattaactgca

Reverse: gttaatgatgatggtgatggtgggatcccat


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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