Tag

Part:BBa_J70046

Designed by: Julie Norville   Group: Knight Lab   (2009-01-22)

6his part for use with BioScaffold parts ATG-6 his-GATCC(glycine serine)-TAA

This 6 His prefix tag can be used with BBa_J70030 (PpiI) in fusing a 6 his tag to the N-terminus of a protein. This part is still in testing. During the BioScaffold part excision, it should be placed in pSB2K4.

Oligos needed for annealing the part and placing it into a EcoRI/SpeI cut vector (such as pSB2K4 that already contains J70030):

Forward: aattcgcggccgcttctagatgCACCATCACCATCATCATggatcctaata

Reverse: ctagtattaggatccATGATGATGGTGATGGTGcatctagaagcggccgcg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 22
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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