Reporter

Part:BBa_J70008

Designed by: Spenser Skates   Group: Knight Lab   (2008-04-30)

Photorhabdus luminescens luciferase operon

The luxCDABE operon of Photorhabdus luminescens can be used as a reporter gene when attached to a promoter and is notably stable at relatively high temperatures, unlike many other luciferases. The operon codes for both the subunits necessary to form the luciferase protein (luxA and luxB) and the enzymes required for the production of the luciferase substrate tetradecanal (luxC, luxD, and luxE).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5280
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2937
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1317
    Illegal SapI site found at 3456


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Categories
Parameters
None