Project

Part:BBa_J64713

Designed by: laura lavery   Group: Voigt Lab   (2007-03-24)


LasR/LasI Inducible (RHLR/RHLI repressible) positive feedback production of 3OC12 HSL

This construct will produce more 3OC12 HSL when the circuit is given an inital amount of 3OC12 and when LasR is present in the system. This construct will also produce RFP as a reporter when expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 406
    Illegal AgeI site found at 1373
    Illegal AgeI site found at 1485
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None