Plasmid

Part:BBa_J64101

Designed by: Jeffrey J Tabor   Group: Voigt Lab   (2007-04-14)


ColE1 biobrick plasmid with backward terminator

ColE1 biobrick plasmid with backward terminator in MCS

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 124
    Illegal suffix found in sequence at 275
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 124
    Illegal SpeI site found at 276
    Illegal PstI site found at 290
    Illegal NotI site found at 130
    Illegal NotI site found at 283
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 124
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 124
    Illegal suffix found in sequence at 276
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 124
    Illegal XbaI site found at 139
    Illegal SpeI site found at 276
    Illegal PstI site found at 290
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None