Coding

Part:BBa_J64027

Designed by: Dan Widmaier   Group: Voigt Lab   (2008-01-16)


sopB Secretion Signal

This part consists of the 168 amino acid secretion signal of sopB in the Salmonella Type III Secretion System (T3S). When fused to a protein of interest this signal, when co-expressed with the cognate chaperone sigE (BBa_J64025), will be directed to the T3S secretion apparatus for export to the extracellular environment. The co-expression of the chaperone is critical for high titers of secreted protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 114
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None