Tag
SicP-SptP

Part:BBa_J64008

Designed by: Dan Widmaier   Group: Voigt Lab   (2008-01-15)


SicP-SptP chaperone-secretion tag pair from Salmonella type III secretion system

This part is the natural implementation of the SicP chaperone (BBa_J64002) and SptP secretion signal (BBa_J64003) from the Salmonella Type III Secretion System (T3S, the Salmonella T3S coding region is referred to as Salmonella Pathogeneity Island 1 [SPI-1]). In the natural implementation the coding regions of SicP and SptP overlap out of frame. This part captures that configuration for use in creating secreted protein / chaperone pairs.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 370
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 370
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 370
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 370
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/localization
Parameters
None