Coding

Part:BBa_J62007

Designed by: John Dueber   Group: Keasling Lab   (2008-07-29)

{SH3 ligand}{SH3 ligand}

two repeats of a 5' glycine-serine linker followed by an SH3 ligand with a Kd=0.1uM affinity for N-terminal murine Crk SH3 domain. The sequence for these two repeats are degenerate ("C" and "A").


This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None