Coding

Part:BBa_J62005

Designed by: John Dueber   Group: Keasling Lab   (2008-07-26)

{HMGS} HMG CoA Synthase

coding sequence for the Saccharomyces cerevisiae enzyme 3-hydroxy-3-methylglutaryl-Coenzyme A Synthase (HMGS) codon optimized for expression in E. coli.

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1177
    Illegal PstI site found at 1405
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1177
    Illegal PstI site found at 1405
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1177
    Illegal PstI site found at 1405
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1177
    Illegal PstI site found at 1405
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None