Coding

Part:BBa_J62002

Designed by: John Dueber   Group: Keasling Lab   (2008-07-26)

{SH3 ligand}

Sequence encoding an N-terminal 5 total amino acid glycine/serine linker and a peptide ligand with a Kd=0.1uM affinity for murine Crk N-terminal SH3 domain. Degenerate sequence "A."

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None