Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Other

Part:BBa_J61017

Designed by: John Anderson   Group: Arkin Lab   (2006-08-11)


Biobrick converter for NotI-part-EcoRI

Inserting a NotI/EcoRI fragment into the PspOMI/MfeI sites of J61017 destroys the restriction sites and introduces standard Biobrick flanking sequence. In the process, the OnRFP cassette is destroyed allowing Red to White screening of colonies.

The registry sequence is blunt relative to XbaI/SpeI.

ApE file:{pSB1A2-Bca1009}

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 641
    Illegal AgeI site found at 753
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None