Plasmid
pAC-LuxGFP
Part:BBa_J61009
Designed by: John Anderson Group: Arkin Lab (2006-08-04)
pAC-LuxGFP
pAC-LuxGFP places GFP under the wildtype Vibrio lux device. It's behavior is described in the cytometry data to the right.
This data, and more on this construct are described in:
<biblio>
- Anderson pmid=16330045
</biblio>
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1733
Illegal EcoRI site found at 2688
Illegal XbaI site found at 588
Illegal XbaI site found at 5084
Illegal PstI site found at 138
Illegal PstI site found at 2498 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1733
Illegal EcoRI site found at 2688
Illegal NheI site found at 2657
Illegal NheI site found at 4242
Illegal PstI site found at 138
Illegal PstI site found at 2498
Illegal NotI site found at 6 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1733
Illegal EcoRI site found at 2688 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1733
Illegal EcoRI site found at 2688
Illegal XbaI site found at 588
Illegal XbaI site found at 5084
Illegal PstI site found at 138
Illegal PstI site found at 2498 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1733
Illegal EcoRI site found at 2688
Illegal XbaI site found at 588
Illegal XbaI site found at 5084
Illegal PstI site found at 138
Illegal PstI site found at 2498
Illegal AgeI site found at 4328
Illegal AgeI site found at 4652 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2781
Illegal BsaI site found at 2959
Illegal BsaI.rc site found at 2423
[edit]
Categories
Parameters
//function/tumorkillingbacteria
//plasmid
//plasmid
chassis | |
copies | |
mcs | Nonstandard |
origin | p15A |
resistance | C |