Part:BBa_J58103

Mutated promoter activated by OmpR-P with the reporter GFP

We have assembled promoter BBa_R0083 to GFP for characterization purposes. That promoter is regulated positively (activated) by the transcription factor OmpR-P (phosphorylated), and it contains only one operator in its sequence, whereas in the natural promoter BBa_R0082 there are three.

The deep components are as follows:

OmpR R0083	B0034	GFP E0040	B0010	B0012

This composite part will be very useful to characterize many cellular biosensors, jointly with the part BBa_J58102.

By Valencia iGEM 2006

A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3).

1 Universidad Politecnica de Valencia, Spain

2 Universitat de Valencia, Spain

3 Ecole Polytechnique, France

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 748