Reporter

Part:BBa_J58101

Designed by: Valencia iGEM 2006   Group: iGEM2006_Valencia   (2006-10-26)

Promoter activated synergistically by cI and CRP with the reporter RFP

This composed part contains a two-regulator AND promoter activated synergistically by cI and CRP proteins:BBa_J58100joined to a generator of the reporter RFPBBa_I13507The deep components are as follows:
pAND
J58100
B0034		mRFP1
E1010
B0010
B0012
With this construction, we obtain a genetic device (not using POPS) that produces RFP when enough levels of cI and CRP are acheived simultaneously.

By Valencia iGEM 2006

A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3).

1 Universidad Politecnica de Valencia, Spain

2 Universitat de Valencia, Spain

3 Ecole Polytechnique, France


Usage and Biology

See comments in BBa_J58100

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 11
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 11
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 11
    Illegal AgeI site found at 687
    Illegal AgeI site found at 799
  • 1000
    COMPATIBLE WITH RFC[1000]

See comments in BBa_J58100


[edit]
Categories
//classic/reporter/pret
Parameters
None