Part:BBa_J58100

AND-type promoter synergistically activated by cI and CRP

We have designed a promoter synergistically activated by two transcription factors. We have used the part BBa_I12007 as starting point, together with the ideas of the paper by Joung et al. Two heterologous prokariotic activators, the bacteriophage lambda cI protein, and the E. coli cyclic AMP receptor protein (CRP), activate transcription synergistically from an artificial promoter bearing binding site for both proteins. Both proteins interact directly with RNA polymerase and bind DNA at two operator sites.

By Valencia iGEM 2006

A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3).

1 Universidad Politecnica de Valencia, Spain

2 Universitat de Valencia, Spain

3 Ecole Polytechnique, France

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 11
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 11
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 11
  
• 1000
  COMPATIBLE WITH RFC[1000]

The CRP binding site contains an XbaI site that could not be removed without compromising the promoter's activity. Avoid using XbaI when using this part. A new part is under development that will have a mutated but functional CRP binding site.