Part:BBa_J56017

sequence containing necessary DNA for controlled chemotaxis

DNA includes switching mechanisms and componenents necssary for directed motility towards aspartate or phenylalanine (does not include wild-type CheW, which may be inserted between restriction sites on opposite side of inversion sequence). Sequence must be placed in strain with all receptors knocked out (tar, tsr, tap, trg, ser) as well as CheW. Switching mechanism is irreversibly activated by arabinose. Strain switches from phenylalanine chemotaxis to aspartate chemotaxis.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 7395
  Illegal SpeI site found at 2824
  Illegal PstI site found at 3761
  Illegal PstI site found at 4213
  Illegal PstI site found at 5336
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1300
  Illegal NheI site found at 5488
  Illegal SpeI site found at 2824
  Illegal PstI site found at 3761
  Illegal PstI site found at 4213
  Illegal PstI site found at 5336
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1239
  Illegal XhoI site found at 5695
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 7395
  Illegal SpeI site found at 2824
  Illegal PstI site found at 3761
  Illegal PstI site found at 4213
  Illegal PstI site found at 5336
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 7395
  Illegal SpeI site found at 2824
  Illegal PstI site found at 3761
  Illegal PstI site found at 4213
  Illegal PstI site found at 5336
  Illegal NgoMIV site found at 5637
  Illegal NgoMIV site found at 6564
  Illegal AgeI site found at 1074
  Illegal AgeI site found at 1340
  Illegal AgeI site found at 3283
  Illegal AgeI site found at 5314
  Illegal AgeI site found at 7017
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 1056
  Illegal SapI.rc site found at 5846