Coding
Part:BBa_J56006
Designed by: Matt Eames Group: iGEM2006_UCSF (2006-10-31)
Codon-optimized signaling domain, cytoplasmic domain of Tar
The codon-optimized cytoplasmic domain of the aspartate receptor Tar, with a single point mutation of EA400 (position 400 in the full sequence), coding for the last 253 amino acids of the full protein. Combined with BBa_J56005, it will encode the full sequence of Tar (EA400). Mutation for orthogonal interaction was discovered in the serine receptor, Tsr (see reference).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 533
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 685
Illegal PstI site found at 533 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 533
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 533
Illegal NgoMIV site found at 834
Illegal AgeI site found at 511 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |