Coding

Part:BBa_J56006

Designed by: Matt Eames   Group: iGEM2006_UCSF   (2006-10-31)

Codon-optimized signaling domain, cytoplasmic domain of Tar

The codon-optimized cytoplasmic domain of the aspartate receptor Tar, with a single point mutation of EA400 (position 400 in the full sequence), coding for the last 253 amino acids of the full protein. Combined with BBa_J56005, it will encode the full sequence of Tar (EA400). Mutation for orthogonal interaction was discovered in the serine receptor, Tsr (see reference).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 533
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 685
    Illegal PstI site found at 533
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 533
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 533
    Illegal NgoMIV site found at 834
    Illegal AgeI site found at 511
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None