Coding

Part:BBa_J56001

Designed by: Matt Eames   Group: iGEM2006_UCSF   (2006-10-13)

CheW with VM108 mutation for orthogonal interaction with Tar EA400

Part contains CheW with valine to methionine mutation at position 108 to facilitate an orthogonal interaction with a Tar chemotaxis receptor with a EA400 mutation. CheW VM108 will have only limited interaction with wild-type Tar. Orthogonal interact was originally revealed only in Tsr (see reference). Sequence is codon-optimized to avoid recombination with similar sequences in the system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 482
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 482
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 482
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 482
    Illegal AgeI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None