Device

Part:BBa_J55001

Designed by: Charles Yoon   Group: iGEM06_Toronto   (2007-10-26)


Produce LacZ alpha in response to AHL

This device generates the LacZ-alpha fragment in the presence of AHL molecules. When transformed into strains producing endogenous LacZ-omega fragments, such as DH5a, the two fragments combine to form the fully functional b-galactosidase enzyme.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1161
    Illegal XhoI site found at 1212
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985


[edit]
Categories
Parameters
None