Deleted
Sample Discontinued
Experience: None
Not Used
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Reporter

Part:BBa_J52999

Designed by: Jelka Pohar   Group: iGEM06_Slovenia   (2006-10-09)


GFP-linker-PEST191

GFP(celeurean)-linker-PEST191, it is a reporter part fused with PEST sequence (accelerated degradation - 2h), for usage in eukariotes.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1242
    Illegal PstI site found at 1256
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1242
    Illegal PstI site found at 1256
    Illegal NotI site found at 7
    Illegal NotI site found at 1249
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1242
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1242
    Illegal PstI site found at 1256
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 780


[edit]
Categories
Parameters
None