Deleted
Sample Discontinued
Experience: None
Not Used
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Coding

Part:BBa_J52222

Designed by: Monika Ciglic   Group: iGEM2006_Slovenia   (2006-09-16)

dnMyD88-linker-rluc

dnMyD88-linker-rluc is made of TIR domain of human MyD88 connected with rluc (which acts as a reporter) through linker that codes for six aminoacids

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1393
    Illegal PstI site found at 1407
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1393
    Illegal PstI site found at 1407
    Illegal NotI site found at 7
    Illegal NotI site found at 1400
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 442
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1393
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 1393
    Illegal PstI site found at 1407
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1003


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Parameters
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