Deleted
Sample Discontinued
Experience: None
Not Used
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Composite

Part:BBa_J52055

Designed by: Ota Fekonja   Group: iGEM2006_Slovenia   (2006-10-12)


NFkappaB promotor + Rluc-linker-PEST191

Part Rluc-linker-PEST191 is joined with inducible promotor.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 864
    Illegal XbaI site found at 16
    Illegal XbaI site found at 879
    Illegal SpeI site found at 836
    Illegal SpeI site found at 2322
    Illegal PstI site found at 850
    Illegal PstI site found at 2336
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 864
    Illegal SpeI site found at 836
    Illegal SpeI site found at 2322
    Illegal PstI site found at 850
    Illegal PstI site found at 2336
    Illegal NotI site found at 7
    Illegal NotI site found at 710
    Illegal NotI site found at 843
    Illegal NotI site found at 870
    Illegal NotI site found at 2329
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 864
    Illegal BglII site found at 797
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 836
    Illegal EcoRI site found at 864
    Illegal XbaI site found at 879
    Illegal SpeI site found at 2322
    Illegal PstI site found at 2336
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 864
    Illegal XbaI site found at 16
    Illegal XbaI site found at 879
    Illegal SpeI site found at 836
    Illegal SpeI site found at 2322
    Illegal PstI site found at 850
    Illegal PstI site found at 2336
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1431
    Illegal SapI site found at 1859


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Parameters
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