Coding

Part:BBa_J52030

Designed by: Jelka Pohar   Group: iGEM06_Slovenia   (2006-10-15)


dnMyD88-GFP-PEST191

dnMyD88-linker-GFP-linker-PEST191 is a part where dnMyD88 (important protein in signaling cascade through TLR4 and TLR5 receptor) is linked to GFP (reporter) and PEST191 sequence that leads towards degradation of protein linked with this sequence. It's estimated half-time is 2h. Both linkers have the same sequence and are 6 aminoacid long protein linker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1194


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Categories
Parameters
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