Composite

Part:BBa_J52024

Designed by: Monika Ciglic   Group: iGEM2006_Slovenia   (2006-10-14)


NFkB + dnMyD88-linker-Rluc-linker-PEST191

This composite part is made of inducible promotor NFkB and dnMyD88 (very important protein in signaling cascade through TLR4) linked to reporter and degradation tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 689
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 776
    Illegal BamHI site found at 1241
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1802
    Illegal SapI site found at 2230


[edit]
Categories
Parameters
None