Coding

Part:BBa_J51001

Designed by: Peter Nguyen   Group: iGEM06_Rice   (2006-10-25)

ComA

Usage and Biology

ComA is the response regulator for the ComQXPA quorum-sensing system of B.subtilis. In the presence of extracellular levels of ComX (a secreted 10-residue modified peptide), the membrane receptor protein ComP phosphorylates ComA, which acts as a transcriptional activator.

For more information on the ComQXPA system, see:

  • Molecular Microbiology (2005) 57(4), 1159–1174.
  • Molecular Microbiology (2000) 37(5), 1159-1171.


For more information on the DNA binding site of ComA, see:

  • J. Bacteriology (1993) 175(10), 3182-3187.


Technical Notes

1. The ComA gene was isolated by PCR from genomic DNA of B.subtilis 168.

2. Due to a large percentage of A+Ts, the primer hybridization sequence was lengthened and the Biobrick sites were correspondingly shortened. Thus, the part does not contain the canonical Biobrick sites (EcoRI-NotI-XbaI, SpeI-NotI-PstI), but are instead shorter BB sites (EcoRI-XbaI, SpeI-PstI)

3. This part contains an SpeI site, which will be removed by site-directed mutagenesis in the future.

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