Part:BBa_J45398

BAT2 & THI3 enzyme generator

BBa_J45398 takes as input a transcriptional signal (PoPS) and produce as output the BAT2 and THI3 enzymes that catalyze production of isoamyl alcohol from the cellular metabolite leucine. Isoamyl alcohol is the precursor to banana odor.

Usage and Biology

BBa_J45008 encodes a branched-chain amino acid transaminase derived from the BAT2 gene from Saccharomyces cerevisiae. BAT2 catalyzes the first step in isoamyl alcohol biosynthesis: conversion of leucine to α-ketoisocaproate via enzymatic reaction 2.6.1.42. BBa_J45009 encodes a branched-chain amino acid transaminase derived from the THI3 gene from Saccharomyces cerevisiae. THI3 catalyzes the second step in isoamyl alcohol biosynthesis: conversion of α-ketoisocaproate to 3-methylbutanal via enzymatic reaction 4.1.1.1.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1187
  Illegal EcoRI site found at 2088
  Illegal EcoRI site found at 2787
  Illegal EcoRI site found at 2973
  Illegal SpeI site found at 1881
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1187
  Illegal EcoRI site found at 2088
  Illegal EcoRI site found at 2787
  Illegal EcoRI site found at 2973
  Illegal SpeI site found at 1881
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1187
  Illegal EcoRI site found at 2088
  Illegal EcoRI site found at 2787
  Illegal EcoRI site found at 2973
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1187
  Illegal EcoRI site found at 2088
  Illegal EcoRI site found at 2787
  Illegal EcoRI site found at 2973
  Illegal SpeI site found at 1881
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1187
  Illegal EcoRI site found at 2088
  Illegal EcoRI site found at 2787
  Illegal EcoRI site found at 2973
  Illegal SpeI site found at 1881
  Illegal AgeI site found at 1051
  
• 1000
  COMPATIBLE WITH RFC[1000]