Device

Part:BBa_J44007

Designed by: Brad Ogden   Group: iGEM2006_Missouri   (2006-08-22)


pLac-RBS-Hin-TT-HixC-pBADrev-HixC-RBS-RE-TT-TetF

This a device that uses Hin to produce Hin recombinase that can flip the promotor pBADrev for Tet resistance; this device can turn on/off the genes for Tet resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 982
    Illegal NheI site found at 1318
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1042
    Illegal BamHI site found at 1464
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1490
    Illegal NgoMIV site found at 1858
    Illegal NgoMIV site found at 2018
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None