Device

Part:BBa_J44006

Designed by: Brad Ogden   Group: iGEM06_Missouri   (2006-08-22)


pLac-RBS-HinLVA-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE

This a device that uses Hin to produce Hin recombinase that can flip the promotor pBAD for Tet resistance; this device can turn on/off the genes for Tet resistance. It uses HinLVA as a degradation tag, which will terminate the flipping of the HixC when the HinLVA is completely degraded.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1145
    Illegal NheI site found at 1357
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1085
    Illegal BamHI site found at 1503
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1529
    Illegal NgoMIV site found at 1897
    Illegal NgoMIV site found at 2057
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None