Composite

Part:BBa_J44004

Designed by: Todd Eckdahl   Group: iGEM2006_Missouri   (2006-08-16)


HixC-pBadrev-HixC-RBS-TetF

This is an expression cassette for Tet resistence. The pBADrev promoter is between two HixC sites, allowing for Hin recombinase to flip the pBADrev promoter. This can turn on and off Tet resistence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 35
    Illegal NheI site found at 371
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 95
    Illegal BamHI site found at 517
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 543
    Illegal NgoMIV site found at 911
    Illegal NgoMIV site found at 1071
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None