Composite

Part:BBa_J44003

Designed by: Todd Eckdahl   Group: iGEM06_Missouri   (2006-08-16)

HixC-pBad-HixC-RBS-TetF

This is an expression cassette for Tet resistence. The pBAD promoter is between two HixC sites, allowing for Hin recombinase to flip the pBAD promoter. Flipping could turn on and off Tet resistence.


Usage and Biology

This intermediate conveys Tetracycline resistance to E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 159
    Illegal NheI site found at 371
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 99
    Illegal BamHI site found at 517
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 543
    Illegal NgoMIV site found at 911
    Illegal NgoMIV site found at 1071
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination/hin
Parameters
None