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Part:BBa_J42040

Designed by: Steve Selinsky   Group: iGEM06_Michigan   (2006-10-30)


recomb. backbone

This nine site polylinker (EcoRI, SacI, NotI, NdeI, ClaI, BamHI, SalI, XhoI, HindIII) serves as the backbone to the nested landing pad project. The polylinker allows for easy double digest and stepwise construction.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NotI site found at 19
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 48
    Illegal XhoI site found at 66
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None