Composite

Part:BBa_J40007

Designed by: Horia Vulpe   Group: iGEM2006_McGill   (2006-11-01)


LacI (LVA) + ECFP (LVA) under luxpR promoter + Repressilator

Polycistronic LacI and ECFP proteins with degradation tags regulated by luxpR promoter (expression up-regulated by LuxR/HSL complex) ligated to I5610 (Elowitz Repressilator). Used as a part in a quorum sensing stabilized repressilator system. Allows to select for both bricks with ampicillin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1228
    Illegal BglII site found at 4389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None