Coding

Part:BBa_J40004

Designed by: Jay Nadeau   Group: iGEM06_McGill   (2006-10-26)


Jun-BetaE-YFPN

This is a fusion of Jun to the beta-autotransporter domain of the immunoglobulin A (IgA) protease of Neisseria gonorrhoea (IgA beta) and to the N-terminus of yellow fluorescent protein (YFP). The IgA beta should target the protein to the cell surface, and the Jun should bind Fos. If the bound Fos contains a C-terminus of a fluorescent protein, fluorescence is expected to result.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 430
    Illegal PstI site found at 1604
    Illegal PstI site found at 1730
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 430
    Illegal PstI site found at 1604
    Illegal PstI site found at 1730
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 82
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 430
    Illegal PstI site found at 1604
    Illegal PstI site found at 1730
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 430
    Illegal PstI site found at 1604
    Illegal PstI site found at 1730
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 1438
    Illegal AgeI site found at 1213
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None