Reporter

Part:BBa_J3901

Designed by: Latin American iGEM team   Group: iGEM2006_LatinAmerica   (2006-10-29)

PrFe-mRFP1

This device coupled the promoters PI and PII of rus operon from Acidithiobacillus ferrooxidans with a monomeric red fluorescent protein (BBa_E1010) used as a signal, showing specific sensorbility response to iron ions presence.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1
    Illegal XbaI site found at 241
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1
    Illegal XbaI site found at 241
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1
    Illegal XbaI site found at 241
    Illegal AgeI site found at 1061
    Illegal AgeI site found at 1173
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/reporter/pret
Parameters
None