Coding

Part:BBa_J37029

Designed by: Jonathan Wells   Group: iGEM06_Imperial   (2006-08-14)


Cre recombinase, enzyme excises sequence between loxP sites

Cre recombinase is an enzyme that catalyzes site-specific recombination between two LoxP sites. Depending on the direction of the LoxP sites, the recombinase can either excise the sequence between the two sites and then fuse the DNA or it can invert the sequence. The part can therefore be used for several functions such as a permanent switch and "flipase".

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 356
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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