Coding

Part:BBa_J37023

Designed by: John Chattaway   Group: iGEM06_Imperial   (2006-07-27)


Immunologically Tagged aiiA

This part is identical to the part BBa_C0060 [1] but we have added an epitope tag to the protein. [http://www.sigmaaldrich.com/Area_of_Interest/Life_Science/Antibody_Explorer/Spotlights/Antibodies_To_Epitope_Tags.html]

This should allow us to accurately mesure the concentration of this protein using ELISA assays and purify and handle the protein.

This is a work in progress and more data will be added when it is finished


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None