Part:BBa_J36843

Streptavidin, single-chain dimer + His6 tag (no start codon)

This part codes for the single-chain dimer of streptavidin protein, without the start codon. Streptavidin protein binds strongly to the biotin molecule, and can be used to bind biotinylated nucleic acids or peptides.

There is a histidine tag added at the end of the coding sequence right before the stop codons. You can probe for this protein with an anti-his(C-term) antibody.

It was used as part of a fusion protein with lipoprotein signal peptide and outer membrane protein A transmembrane domains, to express streptavidin on the outer surface of E. coli. Streptavidin exists naturally as a tetramer, so this dimer form was attempted as a better binder of biotin than a monomeric form on the cell surface.

NOTE: According to sequencing, the SpeI site ACTAGT encountered a mutation to ATTAGT. Also, this part exists currently in pCR-Topo2.1 vector.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 787
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 796
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 217
  Illegal AgeI site found at 751
  
• 1000
  COMPATIBLE WITH RFC[1000]