Coding

Part:BBa_J36842

Designed by: Perry Tsai   Group: iGEM2006_Harvard   (2006-10-29)


Streptavidin, dead (no start codon)

This part codes for the core part of wild-type streptavidin protein, without the start codon. Streptavidin protein binds strongly to the biotin molecule, and can be used to bind biotinylated nucleic acids or peptides. However, this version has been mutated so that it will not bind biotin strongly.

It was used as part of a fusion protein with lipoprotein signal peptide and outer membrane protein A transmembrane domains, to express streptavidin on the outer surface of E. coli. This dead mutant would serve as a type of negative control.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
    Illegal AgeI site found at 133
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None