Coding
KaiB

Part:BBa_J36802

Designed by: Zhipeng Sun, Hetmann Hsieh, Jeffrey Lau, David Ramos   Group: iGEM06_Harvard   (2006-10-30)

KaiB coding region

This part contains the Prochlorococcus (Synechococcus) elongatus PCC7942 KaiB Gene. An extra stop codon was placd at the end of the coding region to ensure translational termination. The physical DNA was sequenced as part of synthesis by GeneArt AG. Note that the coding region is bounded by the BioBricks Primers and is on a non-BioBrick vector pPCR-Script (ampR). The biobricks primers can be cut out along with the KaiB coding sequence with KpnI and SacI. The length of the entire plasmid is 3220bp.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 40
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None