Coding
KaiA

Part:BBa_J36801

Designed by: Zhipeng Sun   Group: iGEM06_Harvard   (2006-10-30)

KaiA coding region

This part contains the coding region of the Prochlorococcus (Synechococcus) elongatus PCC7942 KaiA gene. An extra stop codon is placed at end of the coding sequence to insure translational termination. The physical DNA was sequenced as part of synthesis by GeneArt AG. Note that the coding region is bounded by the BioBricks Primers and is on a non-BioBrick vector pGA4 (ampR). The biobricks primers can be cut out along with the KaiA coding sequence with HindIII and SacI. Length of entire plasmid is 3802bp.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 264
    Illegal XhoI site found at 331
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 394


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Categories
Parameters
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