Composite

Part:BBa_J329010

Designed by: Eric Leaman   Group: Behkam Lab   (2015-08-06)


luxIR Quroum Sensing System I

This is a basic quorum sensing part whereby LuxI will be produced to make AHL, LuxR will be produced to bind with AHL, and LVA-tagged GFP will be expressed in high enough concentrations of LuxR-AHL. The RBS in front of luxI has a predicted TIR of 99 au.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1840
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal BsaI.rc site found at 1989
    Illegal BsaI.rc site found at 2716


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