Signalling

Part:BBa_J329000

Designed by: Eric Leaman   Group: Behkam Lab   (2015-06-02)


Simple lux-based QS system

This part is a basic quorum sensing plasmid whereby LuxI will be produced to make AHL, LuxR will be produced to bind with AHL, and LVA-tagged GFP will be expressed in high enough concentrations of LuxR-AHL.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1821
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal BsaI.rc site found at 1970
    Illegal BsaI.rc site found at 2697


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