Generator

Part:BBa_J3108

Designed by: Karmella Haynes   Group: iGEM06_Davidson   (2006-10-12)


AraC-PC, pLac-HinLVA

Should constitutively express the AraC protein (pBad inhibitor). HinLVA is under the control of pLac. Therefore, HinLVA should be expressed in the presence of IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
//function/recombination/hin
Parameters
o_h
proteinAraC, HinLVA
rbs147
tagLVA