Project

Part:BBa_J24672

Designed by: Azeem Kaka   Group: iGEM06_Brown   (2006-07-24)


Tri-Stable Toggle with Flourescent Reporters

The tri-stable toggle switch can alternate between three gene products through transient induction with either tetracycline, arabinose, or lactose. Following an induction event the system remains at a stable state until another induction event.

This project is a conceptual extension of "Construction of a Genetic Toggle in E. Coli" (Gardner, Collins, Nature vol 403 pg 339 - 342)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3094
    Illegal BglII site found at 8031
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None