Regulatory

Part:BBa_J24669

Designed by: Azeem Kaka   Group: iGEM2006_Brown   (2006-07-24)


Tri-Stable Toggle (Arabinose induced component)

This component of the tri-stable toggle switch is activated by arabinose and inhibits pTetR and pLacI by producing both TetR and LacI LVA-tagged proteins. This component is repressed by AraC. It is unterminated to allow for the addition of target genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3094
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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Categories
Parameters
None