Project

Part:BBa_J24016

Designed by: Jamie Lemon   Group: iGEM2006_Brown   (2006-06-30)


Toggle Switch to Immobilize E. Coli

This part uses a pLac promoter to create motB and also uses an inverter (cI and pcI) to produce LacI. In addition LasI is produced in the motile state, which creates the signalling molecule AI-1. LasR is a transcription factor created in the immobile state. AI-1 and LasR form a complex to initiate transcription from pLasR promoter. We call this the Freeze Machine.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 886
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3181
    Illegal AgeI site found at 448
    Illegal AgeI site found at 3378
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1220


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